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Suppression of HCT had a strong impact on lignin content, monolignol
composition, and interunit linkage distribution in pine TEs. ABSL assays
revealed an up to 42% reduction in lignin content in TEs with suppressed
HCT levels ( Table I ; Wagner et al., 2007 ). Similar or even more substantial
reductions in lignin content were observed in angiosperm species with sup-
pressed HCT levels ( Besseau et al., 2007; Chen et al., 2006 ).
As previously observed in angiosperm species, suppression of HCT also
caused substantial changes in lignin composition in pine TEs. Most notable
was an elevation of H-units released by thioacidolysis, which increased from
trace amounts in wild-type controls to up to 31% in transgenic lines ( Table I ).
This finding is consistent with the role of p-coumaroyl-CoA as a precursor
Fig. 4. Impact of HCT suppression on monolignol biosynthesis in P. radiata.
HCT suppression restricted the biosynthesis of coniferyl alcohol (dashed arrows) and
promoted the biosynthesis of p-coumaryl alcohol (bold) and flavonoids (bold).
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