Molecular Biology of Lignification in Grasses - Botanical Research - page 85

TABLE I
Phenotypes of Mutants and Transgenic Plants Affected in Lignin Pathway

Name of mutants/
genetic modification

Lignin content a Unit content a,b

Gene targets Species Other phenotypes References

4CL Switchgrass Pv4CL1/RNAi Reduced

( 34%)

H > ;G < ;S ¼ - Brown-midrib phenotype

- Higher enzymatic
digestibility

Xu et al. (2011)

CCR Maize Zmccr1/first intron-

transposon

Reduced ( 10%) H < ;G < ;S > - No visual phenotype

- Higher enzymatic
digestibility

Tamasloukht et al.
(2011)

- Sclerenchyma cell shape

Ryegrass LpCCR1-1/RNAi Reduced ( 32%) S/G ¼ - No visual phenotype

- Higher enzymatic
digestibility

Tu et al. (2010)

Rice Snl6/SNP-indel n.d.

Less staining with
phloroglucinol-
HCl

n.d. - Suppression of lesion

mimics/no obvious
phenotype
- Higher enzymatic
digestibility

Bart et al. (2010)

COMT Maize bm3/SNP-indel Reduced G > ;S < ; 5-OH

G > ; pCA < ;
FA ¼

- Brown-midrib phenotype
- Higher enzymatic
digestibility

Lapierre et al.
(1988)
Barri`re et al.
(2004a)

Maize COMT AS-cDNA Reduced ( 28%) G > ;S < ; 5-OH

G > ; pCA <

- Brown-midrib phenotype Piquemal et al.

(2002)

Maize COMT sorghum AS-

cDNA

Reduced ( 20%) n.d. - Brown-midrib phenotype

- Higher enzymatic
digestibility

He et al. (2003)

Maize COMT As-cDNA Reduced ( 14%) H < ;S < ; > 5-

OH-G >

- Brown-midrib phenotype
- Higher enzymatic
digestibility

Pichon et al.
(2006)

(continues)