Parenteral Delivery of Peptides and Proteins - Challenges in Delivery of Therapeutic Genomics and Proteomics - page 587

Table 11.16 CPP for P/P Therapeutics Delivery and Their Characteristics

CPP for P/P Delivery Characteristics Reference

VP22-conjugated Gata4 Successfully used for sustained release of the

transcription factor Gata4 into cardiac cells
in vivo , to control the stress responsiveness of
the heart

[451,492,
493]

Tat-conjugated
antiapoptotic protein
-Bcl-xL

Effectively inhibited apoptosis of an ischemic
neuronal cell in the brain of a stroke model when
given by intraperitoneal route transduces cell
within 1-2 h

[494]

Tat-ODD (oxygen-
dependent
degradation domain)
conjugated caspase 3

Hypoxiccell-specific delivery of caspase 3 [495,496]

Crotamine; MPG (a
synthetic CPP derived
from the SV40 virus)

Shows high affinity for actively proliferating
cells. Promising scope for effective delivery of
chemotherapeutics

[497]

Tat-fused PEA-15
(antiapoptotic protein)

Efficiently transduced pancreatic islets ex vivo
with the prevention of apoptosis of islet cells.
Novel opportunity to improve the viability of
transplantable islets

[498]

TAT gene fused to
a human Cu, Zn-
superoxide dismutase
(Cu, Zn-SOD) gene

Resultant TAT-SOD fusion protein
transduced into the cells with improved
pharmacokinetics than the native proteins.
Effective in replenishing the Cu, Zn-SOD in
various disorders related to this antioxidant
enzyme

[499]

Catalase (CAT) gene
fused to Tat and
arginine-rich peptide

Resultant genetic in-frame TAT-CAT and 9Arg-
CAT fusion proteins, efficiently penetrated
the epidermis as well as the dermis of the
subcutaneous, transduced mammalian cells
in a time- and dose-dependent fashion. Novel
approach to deliver antioxidant enzymes

[500]

Tat fused with heat
shock protein 70
(HSP70)

Effective cytoprotection against cellular stress [501]

Tat-fused human
glutamate
dehydrogenase
(GDH)

Effective in GDH deficiency [502]

VP22-E2 fusion proteins Induced apoptosis in transiently transfected human

papillomavirus (HPV)-transformed cervical
carcinoma cell lines. Suggesting effective local
delivery of VP22-E2 fusion proteins can be used
to treat cervical cancer and other HPV-associated
diseases

[503]

( Continued )