Biomedical Engineering Reference
In-Depth Information
Figure 10.4
Transportation
across CACO-2 cell
monolayer.
CACO-2 cell monolayers have major drug-metabolizing enzymes as well as an array
of transporters found in the intestinal cells
[224]
(
Fig. 10.4
).
In the CACO-2 cell experiment, the apparent permeability coefficient (
P
app
) is
determined by Eq. (10.1):
1
d
d
M
t
⋅
(10.1)
P
app
AC
d
,
0
where
C
d,0
is the initial concentration of drug in the donor side and
M
is the mass
of the drug in the receiver side at the time
t
. The apparent permeability coefficient is
further defined in Eq. (10.2):
1
1
1
1
(10.2)
P
P
P
P
app
aq
mono
F
where
P
F
is the permeability coefficient of the filter support,
P
aq
is the perme-
ability coefficient of the aqueous boundary layer, and
P
mono
is the permeability coef-
ficient of the CACO-2 cell monolayer.
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