Gene Delivery Using Chemical Methods - Challenges in Delivery of Therapeutic Genomics and Proteomics - page 183

Delivery systems that confer higher levels of in vivo gene expression with minimal
toxicity should yield increasingly successful clinical outcomes. In order to achieve
safer and more efficient nonviral gene delivery, researchers are currently focused on
improving present available systems and developing new methods of gene delivery.
Acknowledgment
The authors acknowledge the financial assistance received from the Council for
Scientific and Industrial Research, and TIFAC CORE in NDDS, Government of
India, New Delhi, for providing research facilities to the team.
References
[1] Verma IM, Somia N. Gene therapy—promises, problems and prospects. Nature
1997;389:239-42.
[2] Lehrman S. Virus treatment questioned after gene therapy death. Nature 1999;401:
517-18.
[3] Liu Q, Muruve DA. Molecular basis of the inflammatory response to adenovirus vectors.
Gene Ther 2003;10:935-40.
[4] Sun JV, Anand-Jawa V, Chatterjee S, Wong KK. Immune responses to adeno-associated
virus and its recombinant vectors. Gene Ther 2003;10:964-76.
[5] Donahue RE, Kessler SW, Bodine D, McDonagh K, Dunbar C, Goodman S, et al. Helper
virus induced T cell lymphoma in nonhuman primates after retroviral mediated gene
transfer. J Exp Med 1992;176:1125-35.
[6] Gore ME. Adverse effects of gene therapy: gene therapy can cause leukaemia: no shock,
mild horror but a probe. Gene Ther 2003;10:4.
[7] Behr JP. Synthetic gene-transfer vectors. Acc Chem Res 1993;26:274-8.
[8] Rigby PG. Prolongation of survival of tumour-bearing animals by transfer of “immune”
RNA with DEAE dextran. Nature 1969;221:968-9.
[9] Maes R, Sedwick W, Vaheri A. Interaction between DEAE-dextran and nucleic acids.
Biochim Biophys Acta 1967;134:269-76.
[10] Hashimoto T, Yamaoka T. Polymeric gene carrier. In: Taira K, Kataoka K, Niidome T,
editors. Non-viral gene therapy—gene design and delivery. Tokyo: Springer Verlag; 2005.
p. 35-50.
[11] Laemmli UK. Characterization of DNA condensates induced by poly(ethylene oxide) and
polylysine. Proc Natl Acad Sci USA 1975;72:4288-92.
[12] Wagner E, Cotten M, Foisner R, Birnstiel ML. Transferrin-polycation-DNA complexes:
the effect of polycations on the structure of the complex and DNA delivery to cells. Proc
Natl Acad Sci USA 1991;88:4255-9.
[13] Wu CH, Wilson JM, Wu GY. Targeting genes: delivery and persistent expressions
of a foreign gene driven by mammalian regulatory elements in vivo . J Biol Chem
1989;264:16985-7.
[14] Perales JC, Ferkol T, Beegen H, Ratnoff OD, Hanson RW. Gene transfer in vivo : sus-
tained expression and regulation of genes introduced into the liver by receptor targeted
uptake. Proc Natl Acad Sci USA 1994;91:4086-90.