Gene Delivery Using Chemical Methods - Challenges in Delivery of Therapeutic Genomics and Proteomics - page 166

NH

CH 3

CH 3

HN NH

CH 3

CH 3

NH O

N

HN N N

H

O

H

BGTC

O

H 3 C

O

N

H 3 C

N

DOTIM

HO

N
+
N

L HO

Figure 4.18 Chemical structures of amidinium salt lipids and miscellaneous cationic entities II.

mice for their transfection activity in comparison with 1-2-(oleoyloxyethyl)2-oleyl-3-
(2-hydroxyethyl)imidazolinium chloride (DOTIM) [248] . Compound L of the series,
coformulated with cholesterol or diauroyl-phosphatidyle ethanolamine (DLPE), dem-
onstrated transfection activity similar to DOTIM ( Fig. 4.18 ).

4.4 Lipid Polymer Hybrid Systems or Lipopolymers

Combinations of lipids and polymers, known as lipopolymers, have emerged as a
separate class of nonviral vectors. The ternary complex comprised of DNA, lipid, and
cationic polymer is known as a lipopolyplex. Lipopolyplex has a unique structure
composed of a core of polycation-condensed DNA coated with lipid bilayers.
Lipopolyplexes were investigated for their toxicity and transfection efficiency as