Biomedical Engineering Reference
In-Depth Information
Fig. 1
Multiple subunits of retromer are required for HPV16 but not adenovirus infection. HeLa cells were
transfected with a control siRNA or an siRNA against retromer subunits VPS26, VPS29, or VPS35. After 48 h,
the cells were infected with HPV16-GFP (
black bars
) or adenovirus5-GFP (
gray bars
) at MOI of 0.5 and assayed
for GFP expression 48 h later by fl ow cytometry, with normalization to cells transfected with control siRNA
Fig. 2
Rescue of SV40 infection. Control (Lib1), DNAJB11, DNAJB12, and
DNAJB14 knockdown HeLa cells were stably transduced with a DNAJB11*,
DNAJB12*, or DNAJB14* gene containing a mutation in the shRNA binding site,
as indicated, or empty vector (−). Forty-eight hours after SV40 infection, fl ow
cytometry was used to measure the fraction of cells expressing SV40 large T
antigen. Averaged results from independent experiments are shown. Reproduced
from ref.
19
with permission from the American Society for Microbiology
tendency to produce wide-ranging off-target effects. Despite
these limitations, a number of compounds have found widespread
application in the studies of virus entry. The excellent review by
Engel et al. lists chemicals that could target almost every step of
virus entry [
9
].
