Biomedical Engineering Reference
In-Depth Information
9. In order to fi nd the best settings for object identifi cation, it is
advisable to use CellProfi ler in test run mode. To do this, set up
your pipeline, and then start the test run. While in test run mode,
it is possible to change all parameters within a pipeline and then
immediately visualize the changes in object identifi cation.
Acknowledgments
Our work was supported by grant 31003A-125423 from the Swiss
National Science Foundation. We are grateful to Dr. Cécile
Arrieumerlou (Biozentrum, University of Basel) for her support.
References
1. Schmid EM, McMahon HT (2007) Integrating
molecular and network biology to decode
endocytosis. Nature 448:883-888
2. Howes MT, Mayor S, Parton RG (2010)
Molecules, mechanisms, and cellular roles of
clathrin-independent endocytosis. Curr Opin
Cell Biol 22:519-527
3. Sandvig K, Pust S, Skotland T, van Deurs B
(2011) Clathrin-independent endocytosis:
mechanisms and function. Curr Opin Cell Biol
23:413-420
4. Maldonado-Baez L, Williamson C, Donaldson
JG (2013) Clathrin-independent endocytosis:
a cargo-centric view. Exp Cell Res 319:
2759-2769
5. Maxfield FR, McGraw TE (2004) Endocytic
recycling. Nat Rev Mol Cell Biol 5:
121-132
6. Grant BD, Donaldson JG (2009) Pathways
and mechanisms of endocytic recycling. Nat
Rev Mol Cell Biol 10:597-608
7. Ciechanover A, Schwartz AL, Dautry-Varsat
A, Lodish HF (1983) Kinetics of internaliza-
tion and recycling of transferrin and the trans-
ferrin receptor in a human hepatoma cell line.
Effect of lysosomotropic agents. J Biol Chem
258:9681-9689
8. Stein BS, Sussman HH (1986) Demonstration
of two distinct transferrin receptor recycling
pathways and transferrin-independent receptor
internalization in K562 cells. J Biol Chem
261:10319-10331
9. Klausner RD, Van Renswoude J, Ashwell G,
Kempf C, Schechter AN, Dean A, Bridges KR
(1983) Receptor-mediated endocytosis of
transferrin in K562 cells. J Biol Chem 258:
4715-4724
10. Davis RJ, Corvera S, Czech MP (1986) Insulin
stimulates cellular iron uptake and causes the
redistribution of intracellular transferrin recep-
tors to the plasma membrane. J Biol Chem
261:8708-8711
11. van der Sluijs P, Hull M, Webster P, Male P, Goud
B, Mellman I (1992) The small GTP-binding
protein rab4 controls an early sorting event on
the endocytic pathway. Cell 70:729-740
12. Reiterer V, Grossniklaus L, Tschon T, Kasper
CA, Sorg I, Arrieumerlou C (2011) Shigella
fl exneri type III secreted effector OspF reveals
new crosstalks of proinfl ammatory signaling
pathways during bacterial infection. Cell Signal
23:1188-1196
13. Carpenter AE, Jones TR, Lamprecht MR,
Clarke C, Kang IH, Friman O, Guertin DA,
Chang JH, Lindquist RA, Moffat J et al (2006)
Cell Profi ler: image analysis software for identi-
fying and quantifying cell phenotypes. Genome
Biol 7:R100
14. Kriz A, Schmid K, Baumgartner N, Ziegler U,
Berger I, Ballmer-Hofer K, Berger P (2010) A
plasmid-based multigene expression system for
mammalian cells. Nat Commun 1:120
15. Macia E, Ehrlich M, Massol R, Boucrot E,
Brunner C, Kirchhausen T (2006) Dynasore, a
cell-permeable inhibitor of dynamin. Dev Cell
10:839-850
16. Stein BS, Bensch KG, Sussman HH (1984)
Complete inhibition of transferrin recycling by
monensin in K562 cells. J Biol Chem 259:
14762-14772
Search WWH ::




Custom Search