Biomedical Engineering Reference
In-Depth Information
Fig. 6
PALM microscopy in
A. nidulans
. (
a
) Localization of mEosFP-TeaR at hyphal tip. Conventional wide-
fi eld epifl uorescence microscopy (
top
) and PALM microscopy (
bottom
) are compared. The PALM micrograph
is constructed by 1,000 frames with a time resolution of 50 ms (bar = 1
m). (
b
) Intensity profi le of a TeaR
membrane domain illustrating the increased resolution achieved with PALM. Intensity was measured along
the
green
and
red line
in (
a
). (
c
) Four membrane domains identifi ed by cluster analysis (to be published
elsewhere), setting the threshold on the number of neighboring molecules (ten molecules) within a certain
distance r (50 nm). (
d
) Tracking the dynamic behavior of single molecules for a few seconds until they are
photobleached. Each line indicates the movement of a single TeaR molecule. Separate
colors
indicate dif-
ferent molecules
ʼ
Acknowledgments
We thank Dr. Stefanie Weidtkamp-Peters from the Center for
Advanced Imaging (CAi) at the Heinrich-Heine University
Düsseldorf for critical comments on the manuscript. Our research
was in part fi nanced by grants from the German Science Foundation
through DFG/CONACYT FOR1334.
