Biomedical Engineering Reference
In-Depth Information
Fig. 1
COPII vesicle budding in ER-to-Golgi traffi cking. (
1
) Sec12 catalyzes the
exchange of GDP for GTP on Sar1. (
2
) Sec23/Sec24 heterodimers are recruited.
(
3
) Sec13/Sec31 heterotetramers form a layer around the Sec23/Sec24 com-
plex. (
4
) The COPII vesicle is released from the ER membrane, with the cargo
packaged inside
4. Protease Inhibitors (PI) and Phosphatase Inhibitors (PPI)
(e.g., cocktails of these are available from Sigma).
5. FLAG monoclonal antibody (
see
Note 1
).
6. Normal mouse IgG antibody.
