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The asymptotic values for and F to estimate the asymptotic F-statistics were, for this
case:
mm
+
mf
) (1 - F)/2) + F, = ((
mm
)/4
+ (
mf
)/2, F =
(1 - (1 -
mf
, where
mm
= (/(4 + 2
) and
mf
= ((n - 1)
mm
/n) + (1/4n)).
In fact, the reality could be more complex, because here I consider that generations did
not overlap and adult females were replaced by their female progeny each generation within
each lineage. But, these could be the first simulations to obtain vital data to understand the
genetic and reproductive structure of pink river dolphins. To make these simulations a
program was written by the author with the assistance of O. Suescun.
R
ESULTS
The three sampling points showing the highest average allele number were the lagoons 6,
7 and 8 (3.111
+
0.875, 3
+
1.491, 3.111
+
1.523, respectively), while lagoon 3 presented the
lowest average allele number (1.222
+
0.629). However, the average allele number is
dependent on the sample size, while the expected heterozygosity is not. By using this last
statistic, lagoons 2, 6 and 8 (H = 0.6852
+
0.3963, 0.5599
+
0.2178, 0.5526
+
0.2606,
respectively) presented the highest levels of gene diversity, while sampling localities 1 and 3
registered the lowest levels (H
e
= 0.3333
+
0.3143, 0.333
+
0.4714, respectively). Therefore,
no spatial pattern was found for the geographical disposition of the gene diversity in that
Napo-Curaray river transect. Table 1 displays the different gene diversity statistics by lagoon.
Globally, the average allele number (5
+
2.539) and H
e
(0.5271
+
0.2537), values could be
considered as medium for molecular markers such as microsatellites.
Table 1. Average number of alleles (ana), observed heterozygosity (H
obs
) and expected
heterozygosity (H
exp
) for the nine microsatellites employed in each one of the sampling
points studied and overall.
Population
ana
H
obs
H
exp
beach1
1.67 ± 0.67
0.278 ± 0.342
0.333 ± 0.314
lagoon2
1.89 ± 0.87
0.667 ± 0.408
0.658 ± 0.396
lagoon3
1.22 ± 0.63
0.333 ± 0.471
0.333 ± 0.471
lagoon4
2.67 ± 1.05
0.459 ± 0.303
0.538 ± 0.239
lagoon5
1.89 ± 0.99
0.278 ± 0.342
0.389 ± 0.377
Lagoon6
3.11 ± 0.87
0.459 ± 0.172
0.559 ± 0.218
lagoon7
3.00 ± 1.49
0.294 ± 0.267
0.489 ± 0.316
lagoon8
3.11 ± 1.52
0.456 ± 0.281
0.552 ± 0.260
Total
5.00 ± 2.54
0.405 ± 0.166
0.527 ± 0.254
By means of an exact test using Markov chains, I analyzed possible HWE deviations
within each geographic point sampled (Table 2). Only a very limited number of cases showed
a significant homozygote excess.
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