AMAZON RIVER DOLPHIN POLYMORPHISM AND POPULATION DIFFERENTIATION OF MHC CLASS II PEPTIDES - Biology, Evolution and Conservation of River Dolphins Within South America and Asia - page 122

the consensus sequence. The major divergence was found comparing the consensus sequence
with Inge DQB1-0302, a private allele from the Inírida River in the Orinoco Basin.
Furthermore, the allele Inge DQB1-0102 was the most similar sequence to the consensus one.
The major physical-chemical divergence was produced changing tyrosine-asparagine
(position 37), an aromatic amino acid for a long linear acid (hydrophobic one), and also
changing arginine-glycine (positions 5 and 51).
The highest polymorphism was found in the Orinoco Basin although that basin showed
the major proportion of homozygous animals, meanwhile the Bolivian animals showed the
lowest polymorphism, which is consistent with that observed with other molecular markers.
The average evolutionary divergences were calculated between and within the populations
and the results confirmed the major differences between the Orinoco samples (0.135 - 0.140).
Table 1. Segregate sites on theoretical amino acid sequences for DQB-1 exon 2 locus
identified in Inia .

* 20 * 40 *

Inge DQB1

TERVRFVSSY IYNREEYVRF DSDVGEYRAV TELGRPYAEY WNRQKDILEQ TRAELDT 57

0101

Inge DQB1

........R. .......... .......... .......... .......... R...... 57

0102

Inge DQB1

........R. .......... .......... .......... .........R ....... 57

0103

Inge DQB1

.....Y..R. .......... .......... .......... ..S....... ....... 57

0104

Inge DQB1

........R. .......... .......... .......... .......... R...V.. 57

0105

Inge DQB1

......M.R. .......... .......... S......... .......... R...V.. 57

0106

Inge DQB1

....GY..R. .......... .......... .......... .......... ....V.. 57

0107

Inge DQB1

.......... .......... .......... ......N... ..S...L..R G...... 57

0108

Inge DQB1

.......TR. .......... .......... .......... ........R. R...... 57

0201

Inge DQB1

........R. .......... .......... .......... ........R. R...... 57

0202

Inge DQB1

......M.R. ......FA.. .......... .......... ........R. K...... 57

0203

Inge DQB1

.....YM.R. .......... .......... .......... ........RR R...V.. 57

0204

Inge DQB1

.....Y..R. .......... .......... .......... ........RK ....... 57

0205

Inge DQB1

.....Y..R. .......... .......... .....RA... ..S....... ....V.. 57

0301

Inge DQB1

.....Y.TR. ......FA.. .......... .....RT.K. ..S...L... R...... 57

0302

Inge DQB1

.....Y.TR. .......... .......... ......T... ..S....... ....V.. 57

0303