MicroRNAs as Engineering Targets: Pathway Manipulation to Impact Bioprocess Phenotypes - MicroRNAs as Tools in Biopharmaceutical Production - page 78

Table 5.1 Involvement of miRs in industrially-relevant pathways
microRNA

Reported in
CHO

Affected pathway Effect type Target(s)

miR-1 Yes Apoptosis Pro-apoptotic HSP60, HSP70

miR-1d No Apoptosis Anti-apoptotic ?

miR-7 Yes Cell growth Growth inhibitory EGFR

Apoptosis Anti-apoptotic ?

miR-7b Yes Osmotic stress Inhibition of stress response Fos

let-7 Yes Cell growth Growth inhibitory c-Myc, RAS

let-7c/g Yes Apoptosis Pro-apoptotic Bcl-x L Mcl-1

miR-9 Yes Protein secretion Inhibits protein secretion OC2

miR-10a Yes Apoptosis Pro-apoptotic ?

miR-15a@
miR-16

Yes Apoptosis Pro-apoptotic Bcl-2

Hypoxic stress Possible induction of

apoptosis under hypoxia

?

miR-17-5p@
miR-20a

Yes Cell growth Inhibit/promote growth E2F1

Hypoxic stress Possible induction of

apoptosis under hypoxia

Hif1-alpha

miR-18a Yes Cell growth Promotes growth ?

miR-19a Yes Shear stress Anti-proliferative effect under

laminar shear stress

Cyclin D1

miR-21 Yes Cell growth Growth inhibitory

Anti-apoptotic

CDC25A

Apoptosis Decrease in apoptosis under

laminar shear stress

PDCD4

Shear stress PTEN

miR-23a/b Yes Metabolism Control of glutamine

metabolism

GLS

miR-24 Yes Cell growth Growth inhibitory E2F2, c-MYC

miR-26 Yes Hypoxic stress Decrease pro-apoptotic

signals during hypoxia

?

miR-29b Yes Metabolism Control of branched amino

acids metabolism

BCKD

miR-30 No Apoptosis Pro-apoptotic Ubc9, ITGB3

miR-30d Yes Metabolism Regulation of glucose

metabolism

?

miR-31 Yes Cell growth Growth inhibitory E2F2

Hypoxia Possible resistance to

apoptosis under hypoxia

FIH

miR-34a/b/c Yes Cell growth Growth inhibitory CDK4,6, c-MYC,

CREB, Notch1,
E2F3

Apoptosis Pro-apoptotic Bcl-2, SIRT1

Stress response Possible induction of

apoptosis under stresses

Sp1, Sirt1, Mgst1,
Nrf2

miR-93 Yes Oxidative stress Loss of oxidative stress

defense

Sp1, Sirt1, Mgst1,
Nrf2

miR-96 No Protein secretion Inhibits protein secretion Noc2

miR-107 Yes Hypoxia Decrease pro-apoptotic

signals during hypoxia

?

Metabolism Regulation of glucose

metabolism

?

miR-122 Yes Metabolism Regulation of cholesterol and

lipid metabolism

?