Biomedical Engineering Reference
In-Depth Information
Table 4.1
miRNA expression studies in Chinese hamster ovary (CHO) cells
CHO cell type
Cellular states/culture
conditions
Differentially
transcribed
miRNAs
Detected miRNAs
Profiling method
Probe/primer
design
Reference
CHO-K1
Temperature shift (72 h)
(37
◦
Cto31
◦
C)
26
27.3 % present call
Microarray/qPCR
hsa, mmu
Gammell et al.
2007
CHO-K1
Temperature shift (24 h)
(37
◦
Cto31
◦
C)
10
n/a
qPCR
hsa
Barron et al.
2011
CHO-S
Media nutrient depletion
(depleted vs fresh
media)
70
300
Microarray/qPCR
hsa, mmu, rno,
miRBase
12.0
Druz et al.
2011
54 (16)
a
CHO-K1
Recombinant vs Host
5
Microarray/qPCR
hsa, mmu, rno,
miRBase
10.0
Lin et al. 2011
CHO-DG44
K1 vs DG44
5
350
b
Not specified
Various CHO cell types
n/a
Illumina NGS
Not applicable
Johnson et al.
2011
380
c
CHO-K1
Serum-free adapted vs
18
Illumina NGS
Not applicable
Hackl et al.
2011
CHO-K1SV
serum-dependent
8
CHO-DUKXB-11
Recombinant vs Host
CHO-K1
Growth phases during
batch cultivation
118
199
Microarray/qPCR
hsa, mmu, rno,
miRBase 9.2
Bort et al.
2011
Hsa
homo sapiens
, mmu
mus musculus
, rno
rattus norvegicus
, NGS next-generation sequencing, n/a not available
a
16 miRs selected from 54 candidate miRs with expression detected on microarray with high confidence
b
Including similar isomiR sequences in the count
c
Distinct miRNA sequences, not including isomiR sequences
Search WWH ::
Custom Search