Biomedical Engineering Reference
In-Depth Information
8.7
Conclusions
miRNA manipulation is a viable strategy for cell line engineering. miRNAs can
regulate multiple targets within a pathway, and are readily imitated due to their small
size. As discussed above, synthetic miRNA mimics and inhibitors can be used to
test predicted functions, as well as to screen for desired functions. Stable expression
of miRNAs or miRNA inhibitors, especially from an inducible construct and with
targeted integration into a “safe haven”, can be used to perpetuate the phenotype
for recombinant protein production. Finally, miRNA binding sites may be useful to
restrict transgene expression temporally.
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