MicroRNA Detection Methods for Mammalian Cell Lines and Their Applications in Therapeutic Protein Production - MicroRNAs as Tools in Biopharmaceutical Production - page 101

Table 7.1 Examples of Commercially Available qRT-PCR Platforms for miRNA Detection
Manufacturer RT method qPCR detection Additional Normalization controls Sensitivity of detection Linear range of detec-

method features reported tion reported

Applied
Biosystems

Gene-specific RT with
pooling options

Taqman ®
dual-labeled

Stem-loop
RT
primer

Panel of miRNAs
validated

10 copies of synthetic
miRNA

7-log range

Exiqon Oligo-dT primers with

5 -universal tag and
3 - degenerate anchor

SYBR ® Green LNA Panel of miRNAs

validated

10 copies of synthetic
miRNA

7-log range

Qiagen Oligo-dT primers with

universal tag

SYBR ® Green None re-

ported

Five snoRNAs 2000 copies of

synthetic miRNA*

4-log range

EvaGreen ®

Agilent Adaptor primer with

oligo-dT and universal tag

None re-
ported

U6 snRNA 10 copies of synthetic

miRNA

7-log range

* Reported by Hollingshead et al. ( 2007 ) http://www.genetics.pitt.edu/forms/flyers/miRNArealtimepcrevaluation.pdf
All information is based on manufacturer's websites unless otherwise specified