New Approaches for the Identification of Drug Targets in Protozoan Parasites - International Review of Cell and Molecular Biology - page 362

Table 7.2 Selected examples of in-vitro-screening methods for antiprotozoal compounds—cont'd

Principle Method Organism Reference

Transgenic strains Activity of glucuronidase reporter gene Giardia lamblia ( Müller et al., 2009b )

Activity of beta-galactosidase reporter gene. Neospora caninum ( Howe et al., 1997 ;

Howe and Sibley,
1997 )

Sarcocystis neurona ( Gaji et al., 2006 )

Toxoplasma gondii ( McFadden et al., 1997 )

Quantification of yellow fluorescent protein. Sarcocystis neurona ( Gaji et al., 2006 )

Toxoplasma gondii ( Gubbels et al., 2003 )

Activity of luciferase reporter gene. Plasmodium falciparum ( Cui et al., 2008 )

Activity of luciferase reporter gene, constitutive
or schizont-specific promotor.

Plasmodium berghei ( Franke-Fayard et al.,

2008 )

Vitality Reduction of resazurin. Giardia lamblia ( Bénéré et al., 2007 )

Reduction of tetrazolium. Giardia lamblia ( Wright et al., 1992 )

Quantification of ATP Giardia lamblia ( Chen et al., 2011 )

Quantification of ATP Pneumocystis carinii ( Chen and Cushion,

1994 )

Quantification of mRNA specific for the
parasite in a coculture with host cells.

Neospora caninum ( Strohbusch et al., 2008 )

Cell damage Release of nucleoside hydrolase. Giardia lamblia ( Kang et al., 1998 )