Biology Reference
In-Depth Information
Table 7.2
Selected examples of in-vitro-screening methods for antiprotozoal compounds—cont'd
Principle
Method
Organism
Reference
Transgenic strains
Activity of glucuronidase reporter gene
Giardia lamblia
(
Müller et al., 2009b
)
Activity of beta-galactosidase reporter gene.
Neospora caninum
(
Howe et al., 1997
;
Howe and Sibley,
1997
)
Sarcocystis neurona
(
Gaji et al., 2006
)
Toxoplasma gondii
(
McFadden et al., 1997
)
Quantification of yellow fluorescent protein.
Sarcocystis neurona
(
Gaji et al., 2006
)
Toxoplasma gondii
(
Gubbels et al., 2003
)
Activity of luciferase reporter gene.
Plasmodium falciparum
(
Cui et al., 2008
)
Activity of luciferase reporter gene, constitutive
or schizont-specific promotor.
Plasmodium berghei
(
Franke-Fayard et al.,
2008
)
Vitality
Reduction of resazurin.
Giardia lamblia
(
Bénéré et al., 2007
)
Reduction of tetrazolium.
Giardia lamblia
(
Wright et al., 1992
)
Quantification of ATP
Giardia lamblia
(
Chen et al., 2011
)
Quantification of ATP
Pneumocystis carinii
(
Chen and Cushion,
1994
)
Quantification of mRNA specific for the
parasite in a coculture with host cells.
Neospora caninum
(
Strohbusch et al., 2008
)
Cell damage
Release of nucleoside hydrolase.
Giardia lamblia
(
Kang et al., 1998
)
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