New Approaches for the Identification of Drug Targets in Protozoan Parasites - International Review of Cell and Molecular Biology - page 361

Table 7.2 Selected examples of in-vitro-screening methods for antiprotozoal compounds
Principle Method Organism Reference

Counting by eye Staining of infected cells with cresyl violet.

Counting of cysts.

Toxoplasma gondii ( Müller et al., 2009a )

Counting of trophozoites under the microscope. Giardia lamblia ( Müller et al., 2006 )

Quantification by dyes Staining with methylene blue. Colorimetric assay. Giardia lamblia ( Busatti and Gomes,

2007 )

Staining of parasite DNA in human erythrocytes
by intercalating dye.

Plasmodium falciparum ( Banieck et al., 2007 )

( Duffy and Avery, 2012 )

Immunofluorescence of liver stages in infected
hepatocytes.

Plasmodium sp . ( Gego et al., 2006 )

Quantification after
radioactive labeling

Incorporation of radioactive precursor into DNA,
counting after given time points.

Plasmodium falciparum ( Desjardins et al., 1979 )

( Rottmann et al., 2010 )

Incorporation of radioactive precursor into DNA,
counting after given time points.

Giardia lamblia ( Adagu et al., 2002 )

Quantitative PCR Quantitative real-time PCR for tachyzoite DNA

in infected Vero cells.

Besnoitia besnoitii ( Cortes et al., 2007 )

Quantitative real-time PCR for merozoite DNA
in infected human ileocecal adenocarcinoma
cells

Cryptosporidium parvum ( Arai et al., 2011 )

Quantitative real-time PCR for tachyzoite DNA
in infected fibroblasts.

Neospora caninum ( Esposito et al., 2005 )

Quantitative real-time PCR for tachyzoite DNA
in infected fibroblasts.

Toxoplasma gondii ( Kropf et al., 2012 )

Continued