Biomedical Engineering Reference
In-Depth Information
3 Strain Selection for
Biodiesel Production
Subburamu Karthikeyan
Department of Agricultural Microbiology
Tamil Nadu Agricultural College
Coimbatore, India
CONTENTS
3.1 Bioprospecting ................................................................................................ 17
3.2 Isolation and Characterization of Naturally Occurring Algae ....................... 21
3.3 Isolation Techniques ....................................................................................... 22
3.3.1 Media Configuration ........................................................................... 23
3.3.2 Traditional Methods ............................................................................ 23
3.3.3 Advanced Methods ............................................................................. 27
3.4 Screening Criteria and Methods ..................................................................... 28
3.5 Screening and Selection for Lipid Production ................................................ 31
3.6 Preservation .................................................................................................... 33
3.6.1 Transfer Techniques ............................................................................ 33
3.6.2 Maintenance Conditions ..................................................................... 33
3.6.3 Cryopreservation ................................................................................. 35
3.7 Role of Repositories ........................................................................................ 37
3.8 Concluding Remarks ...................................................................................... 37
References ................................................................................................................ 38
3.1 BIOPROSPECTING
Bioprospecting is the collection of biological material and the exploitation of its
molecular, biochemical, and/or genetic content for the development of a commer-
cial product. Precisely, bioprospecting relies on the endowment of a bioresource,
a  stock  of novel biodiversity. Bioprospecting is a time-consuming process, where
new products and markets must be identified, and a compound that covers com-
mercial demands and social needs must be discovered. Algae are ubiquitous and
have been evolving as primary biomass producers on the Earth for billions of years.
Exploring this existing, self-maintaining, and diverse life form offers a rich base
for global biotechnological innovations. Indigenous species are well adapted to pre-
vailing regional abiotic and biotic factors, and further local strains provide an ideal
platform for additional strain improvement and process optimization. Many algal
species remain unknown or unexplored in science, giving logical attention to explore
17
 
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