Micropatterning of Substrates and Cells - Introduction to BioMEMS - page 57

Cover glass

Membrane receptor

c

a

Cell

b

Biotinylated peptide
BSA

Streptavidin

Photobleached
fluoroscein

Biotin-4-fluoroscein

Glass-
bottomed
dish

d

Objective

20 µm

Camera port

Microscope Spatial filter Collimated

diode

10 µm

FIGURE 2.11 Photopatterning.of.proteins.using.photobleaching-induced.adsorption..(From.Jonathan.
M..Bélisle,.Dario.Kunik,.and.Santiago.Costantino,.“Rapid.multicomponent.optical.protein.pattern-
ing,”. Lab Chip . 9,. 3580-3585,. 2009.. Reproduced. with. permission. from. The. Royal. Society. of.
Chemistry.)

a c

100% 50% 25% 0%

1.0 Biotin binding

10 µm

0.0

Position

b d

1.0

Neurite

Avidin
BSA

Protein
matrix

1 µm

0.0

4 7 4 7

pH

FIGURE 2.12 Selective.in.situ.photo-cross-linking.of.albumin..(From.Bryan.Kaehr,.Richard.Allen,.
David.J..Javier,.John.Currie,.and.Jason.B..Shear,.“Guiding.neuronal.development.with.in.situ.micro-
fabrication,”. Proc. Natl. Acad. Sci. U. S. A. .101,.16104,.2004..Copyright.(2004).National.Academy.
of.Sciences,.U..S..A..Figure.contributed.by.Jason.Shear.)