Biomedical Engineering Reference
In-Depth Information
Hepatocytes
+Fibroblasts
100 µm
FIGURE A.4
Micropatterned.coculture.
(a)
(b)
(c)
Z
30 min
24 h
Y
X
FIGURE A.5
Micropatterning.cellular.adhesiveness.
Ater 30 minutes, the unattached cells are removed, and the cell culture surface appears like the
micrograph on
Figure A.5b
, with four blue cells attached on the central “X” region. Ater 24
hours, the cells have proliferated and have been able to attach and migrate onto the “Y” region
(but not onto the “Z” region!), with the result that the island looks approximately like that in
Figure A.5c
.
Provide one name of surface coating/grat and cell type for which this patterning scheme
would work and that would be consistent with
all three
images; please
briely
explain why you
chose it:
Cell type = …………………Why?
X = ………………………… Why?
Y = ………………………… Why?
Z = ………………………… Why?
Exercise A.2.5.
he picture in
Figure A.6
shows two pairs of cells attached to a layer of ECM
protein (on a substrate of your choice) and separated by a chemically grated layer containing
PEG
ECM protein
Endothelial cells
20 µm
FIGURE A.6
PEG.micropattern.
