Tissue Microengineering - Introduction to BioMEMS - page 389

Microwell crosssection

a b

Microwells

PDMS
Glass

1

Fibroblast layer

MEF

2

hES

ES cells

3

FIGURE 7.18 Formation.of.embryoid.bodies.in.PDMS.microwells..Scale.bar.is.200.μm..(From.Ali.
Khademhosseini,.Lino.Ferreira,.James.Blumling.III,.Judy.Yeh,.Jeffrey.M..Karp,.Junji.Fukuda,.and.
Robert.Langer,.“Co-culture.of.human.embryonic.stem.cells.with.murine.embryonic.ibroblasts.on.
microwell-patterned.substrates,”. Biomaterials ,.27,.5968-5977,.2006..Reprinted.with.permission.
from.Elsevier..Figure.contributed.by.Ali.Khademhosseini.)

a b

40 µm

UV light PDMS mold

PEG monomer

75 µm

PEG microstructures

Glass

ES cells

100 µm

EBs

150
µm

Z

Y

X X

FIGURE 7.19 Formation.of.embryoid.bodies.in.PEG.microwells..(From.Jeffrey.M..Karp,.Judy.Yeh,.
George.Eng,.Junji.Fukuda,.James.Blumling,.Kahp-Yang.Suh,.Jianjun.Cheng,.Alborz.Mahdavi,.Jeffrey.
Borenstein,.Robert.Langer,.and.Ali.Khademhosseini,.“Controlling.size,.shape.and.homogeneity.of.
embryoid.bodies.using.poly(ethylene.glycol).microwells,”. Lab Chip ,.7,.786-794,.2007..Reproduced.
with.permission.from.The.Royal.Society.of.Chemistry.)