Tissue Microengineering - Introduction to BioMEMS - page 377

3-D laser patterning of agarose gels
Benzophenone-Mal-[ 14 C]-CDPGYICSR a b

Underivatized 0.5%
agarose gel

b

CDPGYIGSR-derivatized agarose gel

500 µm

2.5 mm

FIGURE 7.2 The. 3-D-patterned. laser. immobilization. of. cell-attachment. peptides. in. hydrogels..
(From. M.. Borkenhagen,. J.-F.. Clémence,. H.. Sigrist,. and. P.. Aebischer,. “Three-dimensional. extra-
cellular.matrix.engineering.in.the.nervous.system,”. J. Biomed. Mater. Res. ,.40,.392-400,.1998..
Reprinted.with.permission.from.John.Wiley.and.Sons.)

a c

Bulk
hydrogel

Crosslinked
protein

Coverglass

Objective

Fluorescein-labeled
BSA helices in HA gels

b

Fluorescein-labeled BSA helices in HA gels

d

DRGs on IKVAV helices

40 µm

50 µm

Confocal
stack

FIGURE 7.3 The.3-D.patterning.of.DRG.neurons.using.multiphoton.lithography..(From.Stephanie.
K..Seidlits,.Christine.E..Schmidt,.and.Jason.B..Shear,.“High-resolution.patterning.of.hydrogels.in.
three. dimensions. using. direct-write. photofabrication. for. cell. guidance,”. Adv. Funct. Mater .,. 19,.
3543-3551,.2009..Reprinted.with.permission.from.Wiley.)