Biomedical Engineering Reference
In-Depth Information
a
b
(1)
(2)
Spore
Appressorium
1 µm
Leaf epidermis
Guard cell
of stomata
(3)
Other infection
structures
(4)
10 µm
100
80
60
40
20
0
0.0 0.2 0.4 0.6
Ridge height (µm)
d
c
Appressorium formation
No appressorium formation
Ridge height 0.5 µm
Ridge height 5 µm
0.8 1.0 1.2 1.4
10 µm
FIGURE 6.79
The.role.of.leaf.surface.microtopography.in.fungal.infection..(From.Harvey.C..Hoch,.
Richard. C.. Staples,. Brian. Whitehead,. Jerry. Comeau,. and. Edward. D.. Wolf,. “Signaling. for. growth.
orientation.and.cell.differentiation.by.surface.topography.in.uromyces,”.
Science
.235,.1659-1662,.
1987..Figure.contributed.by.Harvey.Hoch.)
Flow
in
Flip
Top piece
Bottom piece
Assembled device
a
Inlet
tubing
Agar/alginate
block
PDMS
PDMS
Glass
Alignment
posts
Alignment
holes
Arabidopsis root
Clamp
(not shown)
Stream
posts
Clamp
Media
Stimulant
Media
Flow profile at the start
24 hours later
t = 0'
c
b
d
Root
50 µm
Posts
t = 5'
Auxin
stream
t = 10'
t = 20'
50 µm
50 µm
FIGURE 6.80
Root.in.a.channel:.plant.on.a.chip..(From.Matthias.Meier,.Elena.M..Lucchetta,.and.
Rustem.F..Ismagilov,.“Chemical.stimulation.of.the.
Arabidopsis thaliana
.root.using.multi-laminar.low.
on.a.microluidic.chip,”.
Lab Chip
.10,.2147-2153,.2010..Reproduced.with.permission.from.The.
Royal.Society.of.Chemistry.)
