Biomedical Engineering Reference
In-Depth Information
MAP-5 (axons)
1
2
3
4
Mask
1
2
3
4
100 µm
50 µm
MAP-2 (dendrites)
FIGURE 6.58
Determination. of. neuronal. polarity. using. SAMs.. (From. David. A.. Stenger,. James. J..
Hickman,.Karen.E..Bateman,.Melissa.S..Ravenscroft,.Wu.Ma,.Joseph.J..Pancrazio,.Kara.Shaffer,.
Anne.E..Schaffner,.David.H..Cribbs,.and.Carl.W..Cotman,.“Microlithographic.determination.of.axonal/.
dendritic. polarity. in. cultured. hippocampal. neurons,”.
J. Neurosci. Methods
. 82,. 167-173,. 1998..
Figure.contributed.by.David.Stenger.)
a
3 mm
b
β-tubulin
MP-cGMP
Axon
DAPI
Adsorbed protein patterns
Coverslip
Negative control
pattern
(BSA +
cGMP
)
Cell body
pattern
(
PLL
+
cGMP
)
Axonal guidance
pattern
(BSA +
cAMP
)
30 µm
Cell body
FIGURE 6.59
Neuronal. polarization. directed. by. cAMP/cGMP. micropatterns.. (From. J.. Tanner.
Nevill,. Alexander. Mo,. Branden. J.. Cord,. Theo. D.. Palmer,. Mu-ming. Poo,. Luke. P.. Lee,. and. Sarah.
C..Heilshorn,.“Vacuum.soft.lithography.to.direct.neuronal.polarization,”.
Soft Matter
.7,.343-347,.
2011..Reproduced.with.permission.from.The.Royal.Society.of.Chemistry.)
