Biomedical Engineering Reference
In-Depth Information
a
N
b
c
T
A
P
Eye
Tectum
Stop zone
Nasal axons
Temporal axons
100 µm
FIGURE 6.51
Micropatterned. discrete. ephrin. gradients. to. simulate. the. optic. tract. development..
(From.Anne.C..von.Philipsborn,.Susanne.Lang,.Jürgen.Loeschinger,.André.Bernard,.Christian.David,.
Dirk.Lehnert,.Friedrich.Bonhoeffer,.and.Martin.Bastmeyer,.“Growth.cone.navigation.in.substrate-
bound. ephrin. gradients,”.
Development
. 133,. 2487-2495,. 2006.. Figure. contributed. by. Friedrich.
Bonhoeffer.and.Martin.Bastmeyer.)
Array of
filling points
Culture dish
a
Silicon wafer
b
Flow
PDMS cuboid
Printed
gradient
Silicon matrix
Parallel channel
system
Laminin solution
∆Eph
∆Fc
Increasing ephrin A5
concentrations
Axon
outgrowth
Merge
Steep gradient
Shallow gradient
Steep gradient
No gradient
d
e
f
g
h
c
50 µm
Temporal axons
Retinal explant
Temporal axons
Temporal axons
Temporal axons
Nasal axons
FIGURE 6.52
Axon.guidance.by.microluidically.patterned.discrete.gradients..(From.Susanne.Lang,.
Anne. C.. von. Philipsborn,. André. Bernard,. Friedrich. Bonhoeffer,. and. Martin. Bastmeyer,. “Growth.
cone.response.to.ephrin.gradients.produced.by.microluidic.networks,”.
Anal. Bioanal. Chem.
.390,.
809-816,.2008..Figure.contributed.by.Friedrich.Bonhoeffer.and.Martin.Bastmeyer.)
