Biomedical Engineering Reference
In-Depth Information
(a)
400 µm
(b)
(c)
(d)
100 µm
y
Sink
0 µM
Gel
Center
Gel
Source
MeAsp
100 µM
x
0 min
4 min
8 min
(e)
(f )
(g)
y
0-20 min tracks
1.0
1.0
0.014
0.012
0.010
0.008
0.006
0.004
0.002
0.000
300
0.8
0.8
0.6
0.6
200
0.4
0.4
100
0
100
200
y
(µm)
300
400
0.2
0.2
CMC = average position of cells
with respect to center
of channel
t = 20 min
0.0
0.0
100 µm
x
0
50 100 150 200 250 300 350 400
0 2 4 6 8 10
Time (min)
12 14 16 18 20 22
y
position (µm)
FIGURE 6.37
Logarithmic.sensing.of.chemotactic.gradients.by.bacteria..(From.Yevgeniy.V..Kalinin,.
Lili.Jiang,.Yuhai.Tu,.and.Mingming.Wu,.“Logarithmic.sensing.in.
Escherichia coli
.bacterial.chemo-
taxis,”.
Biophys. J.
.96,.2439-2448,.2009..Reprinted.with.permission.from.Elsevier.)
Source
channel
Test
channel
Sink
channel
a
PDMS
Agarose
Glass
Design 1
Design 2
Design 3
0.2
b
Source
1 mm
0.15
0.1
s
0.05
Sink
0
0
5
10
15
s (mm)
0.4
c
1 mm
Source
0.3
0.2
s
0.1
Sink
0
0
2
4
6
8
s (mm)
FIGURE 6.38
Agarose.arbitrary.gradient.generator.for.bacterial.chemotaxis..(From.Tanvir.Ahmed,.
Thomas. S.. Shimizu,. and. Roman. Stocker,. “Bacterial. chemotaxis. in. linear. and. nonlinear. steady.
microluidic.gradients,”.
Nano Lett.
.10,.3379-3385,.2010..Figure.contributed.by.Roman.Stocker.)
