Cell-Based Chips for Biotechnology - Introduction to BioMEMS - page 264

(c)

(a) (b)

Cell flow into traps Cell flow diverted

PDMS PDMS

Streamlines
diverted

Streamlines

30 µm

Glass Glass

FIGURE 5.24 Arrays.of.hydrodynamic.cell.traps..(From.Dino.Di.Carlo,.Nima.Aghdam,.and.Luke.P..
Lee,.“Single-cell.enzyme.concentrations,.kinetics,.and.inhibition.analysis.using.high-density.hydro-
dynamic.cell.isolation.arrays,”. Anal. Chem. .78,.4925-4930,.2006..Reprinted.with.permission.of.
the.American.Chemical.Society.)

a b c d

Load green cells
'up'

Transfer cells
'down'

Load red cells
'down'

Back-side
capture cup

50 µm

Front-side
capture cup

-0.1

f

Support
pillar

20 µm

0

5

e

10

15

200 µm

20

FIGURE 5.25 Microluidic.control.of.cell.pairing.and.fusion..(From.Alison.M..Skelley,.Oktay.Kirak,.
Heikyung.Suh,.Rudolf.Jaenisch,.and.Joel.Voldman,.“Microluidic.control.of.cell.pairing.and.fusion,”.
Nat. Methods .6,.147-152,.2009..Adapted.with.permission.of.the.Nature.Publishing.Group.)