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hCG bound anti-hCG
antibody-enzyme conjugate
Positive test
Positive test
a
Monoclonal
anti-hCG Ab-enzyme
conjugates
b
c
Capillary action
hCG
hormone
R
T
C
Polyclonal
anti-hCG Ab
Anti-mouse
Ab
Dye substrate
Positive test
Positive test
e
Positive test
d
f
Polyclonal
antibodies
Anti-mouse
antibody
Positive test
Positive test
g
h
Conjugated
enzyme
Dye
substrate
Test zone: positive
Control zone: positive
FIGURE 4.9 Home.pregnancy.test.(Adapted.from.“ The HCG Pregnancy Test: How It Works ,”.a.video.developed.by.Susana.Maria.Halpine.for.the. Kuby
Immunology .Web.site..Copyright.(2000).W.H..Freeman.and.Sumanas,.Inc.)
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