Microfluidics - Introduction to BioMEMS - page 204

Picoliter volume

a b c

Dye

Valve 1

Suction (open valve 1)

100 µm

9 nL chambers 300 µm

Picoliter
trapping

(After
closing
valve 1)

3.5 pL chambers

300 µm

BSA Fibronectin

30 µm

FIGURE 3.108 Microstructured.membranes.and.microvalves.for.trapping.luids.and.for.substrate.
patterning..(a;.from.Hsu,.C.-H.,.Chen,.C.,.and.Folch,.A..“Microluidic.devices.with.tunable.micro-
topographies,”. Appl. Phys. Lett. 86,.023508,.2005..Figure.contributed.by.Chia-Hsien.Hsu;.b, from.
Siva. A.. Vanapalli,. Daniel. Wijnperle,. Albert. van. den. Berg,. Frieder. Mugeleac,. and. Michel. H.. G..
Duits,.“Microluidic.valves.with.integrated.structured.elastomeric.membranes.for.reversible.luidic.
entrapment. and. in. situ. channel. functionalization,”. Lab Chip . 9,. 1461-1467,. 2009.. Reproduced.
with.permission.from.The.Royal.Society.of.Chemistry;.c,.from.David.Cate,.Nianzhen.Li,.and.Albert.
Folch,.“Microvalve-addressable.picoliter.chambers.for.single-molecule.enzymology,”. Proceedings of
MicroTAS 2008 ,.San.Diego,.2008..Figure.contributed.by.David.Cate.)

a

12.0 mm

e 0 s 0.1 s

Connecting
air channels

12.0 mm

ø6.5 mm

3.0 mm

0.2 s 0.3 s

Mixing chamber
Fluidic channels

b

PDMS membrane
Beads

Air injection

c Air chamber

0.5 s 0.7 s

Air release

d

FIGURE 3.109 Vortex-type. micromixer.. (From. Sung-Yi. Yang,. Jr-Lung. Lin,. and. Gwo-Bin. Lee,. “A.
vortex-type. micromixer. utilizing. pneumatically. driven. membranes,”. J. Micromech. Microeng. . 19,.
035020,.2009..Reproduced.with.permission.from.the.Institute.of.Physics..Figure.contributed.by.
Gwo-Bin.Lee.)