Biomedical Engineering Reference
In-Depth Information
a
b
1 injection
Multiplexer
c
d
4 injections
Pump 3 sec
FIGURE 3.105
Automated.combinatorial.mixer.based.on.microvalves..In.all.images,.the.diameter.of.
the.mixing.ring.is.1.5.mm..(a).The.peristaltic.pump.pneumatic.channels.are.illed.with.yellow.dye.
and.the.multiplexer.channels.are.illed.with.black.dye..(b).Injection.of.~250.pL.of.blue.dye.into.the.
rotary.mixer..(c).Color.gradient.formed.by.consecutive.injections.of.blue,.green,.yellow,.and.red.dyes.
into.the.rotary.mixer..(d).Pumping.around.the.ring.for.3.seconds.results.in.complete.mixing..(From.
Carl.L..Hansen,.Morten.O..A..Sommer,.and.Stephen.R..Quake,.“Systematic.investigation.of.protein.
phase.behavior.with.a.microluidic.formulator,”.
Proc. Natl. Acad, Sci. U. S. A.
.101,.14431-14436,.
2004.. Copyright. (2004). National. Academy. of. Sciences,. U.S.A.. Figure. contributed. by. Stephen.
Quake.)
a
Filling while valve set #1 open (set #2 closed)
Water
Fluorescein
Valve sets #1 and #2 closed
b
0
255
Mixing while valve set #2 open (set #1 closed)
c
d
Mixing complete, valve sets #1 and #2 closed
0
255
FIGURE 3.106
Metering. of. nanoliter-scale. volumes. in. a. micromixer. using. microvalves.. (From.
Nianzhen.Li,.Chia-Hsien.Hsu,.and.Albert.Folch,.“Parallel.mixing.of.photolithographically.deined.
nanoliter. volumes. using. elastomeric. microvalve. arrays,”.
Electrophoresis
. 26,. 3758-3764,. 2005..
Figure.contributed.by.Nianzhen.Li.)
