Biomedical Engineering Reference
In-Depth Information
Image line
Common
inlet/outlet
Individual wells
Microfluidic network
Inlet/outlet holes
FIGURE 3.22
“Tubeless”. microluidics:. a. 96-well. plate. incorporating. embedded. microchannels..
Serpentine.low.channel.patterns.are.used.to.ensure.an.equal.length.low.path.from.each.analyte.
well.to.the.common.well..(From.Charles.J..Choi.and.Brian.T..Cunningham,.“A.96-well.microplate.
incorporating.a.replica.molded.microluidic.network.integrated.with.photonic.crystal.biosensors.for.
high. throughput. kinetic. biomolecular. interaction. analysis,”.
Lab Chip
. 7,. 550,. 2007.. Reproduced.
with.permission.from.The.Royal.Society.of.Chemistry.)
a
c
d
Inlet
b
1 mm
FIGURE 3.23
Vacuum.manifold.for.world-to-chip.interface..(From.Gregory.A..Cooksey,.Anne.L..Plant,.
and.Javier.Atencia,.“A.vacuum.manifold.for.rapid.world-to-chip.connectivity.of.complex.PDMS.micro-
devices,”.
Lab Chip
.9,.1298,.2009..Reproduced.with.permission.from.The.Royal.Society.of.Chemistry.)
wetting and illing). We have already seen one way to make a hydrophobic channel hydrophilic
which is to chemically modify the surface using a form of oxidation, such as exposure to an oxy-
gen plasma (dry) or a solution containing an oxidizing agent (wet). Alternatively, an intrinsically
hydrophobic surface can be coated with a polymer (synthetic or natural) that adsorbs to it and,
in turn, exposes a more hydrophilic surface: Pluronics [which are triblock copolymers known as
