Micropatterning of Substrates and Cells - Introduction to BioMEMS - page 91

25 µm

FIGURE 2.46 Cell.patterning.using.DNA.barcodes..(From.Erik.S..Douglas,.Ravi.A..Chandra,.Carolyn.
R..Bertozzi,.Richard.A..Mathies,.and.Matthew.B..Francis,.“Self-assembled.cellular.microarrays.pat-
terned.using.DNA.barcodes,”. Lab Chip .7,.1442-1448,.2007..Reproduced.with.permission.from.
The.Royal.Society.of.Chemistry.)

used for patterning several cell types simultaneously if diferent antibodies to selected membrane
proteins of each cell type featuring small cross-reactivities can be produced in a practical manner.
Why limit ourselves to immunocapture? Indeed, Richard Mathies, Matthew Francis, and
coworkers at the University of California at Berkeley have decorated cells with DNA strands
(which they termed “DNA barcodes”) and used patterns of complementary DNA immobilized
on the surface to capture cells in selected locations ( Figure 2.46 ).

2.6.4.2 In Situ Microfabrication of Protein Structures
In 2004, a group led by Matsuhiko Nishizawa from Tohoku University in Sendai, Japan, dem-
onstrated that physisorbed albumin can be electrochemically removed from the substrate on

Microelectrode Microelectrode

a b

X -

X 2

~5 µm

HXO

Scan

Albumin

500 µm

100 µm

FIGURE 2.47 Selective.electrochemical.inactivation.of.albumin..(From.Hirokazu.Kaji,.Masamitsu.
Kanada,.Daisuke.Oyamatsu,.Tomokazu.Matsue,.and.Matsuhiko.Nishizawa, “Microelectrochemical.
approach. to. induce. local. cell. adhesion. and. growth. on. substrates,” Langmuir . 20 , 16-19,. 2004..
Figure.contributed.by.Matsuhiko.Nishizawa.)