Micropatterning of Substrates and Cells - Introduction to BioMEMS - page 88

Cell

a

Cystein-labeled
peptide
Heterobifunctional
crosslinker

S S S S

NH NH NH NH

Si Si Si Si Si Si Si Si Si

b 24 h

50 µm

FIGURE 2.43 Neuronal.micropatterns.using.cell-adhesion.peptides..(From.Mieko.Matsuzawa,.Paivi.
Liesi,.and.Wolfgang.Knoll,.“Chemically.modifying.glass.surfaces.to.study.substratum-guided.neu-
rite.outgrowth.in.culture,”. J. Neurosci. Methods .69,.189-196,.1996..Reprinted.with.permission.of.
Elsevier.)

a e

PDMS

1) O 2 plasma treatment:
2) Inked with EG 6 SH.

Fibroblasts

100 µm

b

PDMS

f

Gold

Si Endothelial

cells

100 µm

Microstamping

c

EG 6 SH SAM

RADSC-14

Si

EG 6 SH

Immersion in
RADSC-14

d

Si

FIGURE 2.44 Patterning.of.cell-adhesion.peptides.by.microstamping..(From.Shuguang.Zhang,.Lin.
Yan,. Michael. Altman,. Michael. Lässle,. Helen. Nugent,. Felice. Frankel,. Douglas. A.. Lauffenburger,.
George.M..Whitesides,.and.Alexander.Rich,.“Biological.surface.engineering:.A.simple.system.for.
cell.pattern.formation,”. Biomaterials .20,.1213-1220,.1999..Reprinted.with.permission.of.Elsevier..
Figure.contributed.by.George.Whitesides.)