Micropatterning of Substrates and Cells - Introduction to BioMEMS - page 84

2.6.2.5 Selective Microluidic Delivery of Proteins
As seen in Section 1.6.4, ECM templates may be deposited on the surface directly from the sol-
uble phase using a microluidic device that is sealed on the surface of interest, an approach that
may be considered the “negative” of microstamping (the protein is deposited where the stamp
does not contact the surface). he technique can be used to create micropatterns of peptides,
DNA, organic polymers, ceramics, proteins, etc.

Protein
solution

PS

PMMA

50 µm

Protein
template

Au

Glass

Cell
attachment

FIGURE 2.38 Cellular.micropatterns.using.microluidic.networks..(From.Albert.Folch.and.Mehmet.
Toner,.“Cellular.micropatterns.on.biocompatible.materials,”. Biotechnol. Prog. 14, 388-392,.1998..
Figure.contributed.by.the.author.)

Voltage waveform

Deformable piezo crystal

Inkjet-deposited
collagen/PDL mixture

Membrane

Ink inlet

Chamber

Motion of ink-jet print head

Orifice

8-10 DIV

Ink droplets
Ink pattern and substrate

100 µm

FIGURE 2.39 Cellular. micropatterns. using. inkjet. printing.. (From. Neville. E.. Sanjana. and. Sawyer.
B.. Fuller,. “A. fast. lexible. ink-jet. printing. method. for. patterning. dissociated. neurons. in. culture,”.
J. Neurosci. Methods .136,.151-163,.2004..Reprinted.with.permission.of.Elsevier..Figure.contrib-
uted.by.Neville.Sanjana.and.Sawyer.Fuller.)