Biomedical Engineering Reference
In-Depth Information
Agarose
Hepatocytes
50 µm
FIGURE 2.31
Coninement.of.cells.using.micromolded.agarose.hydrogel.micropatterns..(From.Albert.
Folch.and.Mehmet.Toner,.“Cellular.micropatterns.on.biocompatible.materials,”.
Biotechnol. Prog.
14,
388-392,.1998..Figure.contributed.by.the.author.)
border of the island for several days (
Figure 2.31
). he same technique was later applied by oth-
ers to produce islands of single cells.
In 1998, Takehisa Matsuda and coworkers at the National Cardiovascular Center Research
Institute in Osaka, Japan were able to synthesize a thiolated poly(vinyl alcohol) (SH-PVA) hydro-
gel which can be straightforwardly immobilized on gold in aqueous solution (
Figure 2.32
). hey
a
b
Gold
1
Thiolated PVA
Metal mask
2
Metal mask
SH
SH
SH
PET
SH
200 µm
SH
(I) Gold
evaporation
SH
SH
SH
SH
Deposited gold
SH
SH
SH
PET
3
Au
Au
4
Gel formation
(by disulfide bonding)
(II) Coating of
thiolated PVA
Drying
200 µm
Thiolated PVA
SS
SH
Day 2
SS
SS
SS
PVA
SS
6
SH
(III) Air drying
and rinsing
S
S
S
S
Cells on PET
PVA hydogel
Fixation of gel
(by chemisorption of thiol
group on gold)
Control (no PVA)
(IV) EC seeding
and culturing
5
Cells
200 µm
FIGURE 2.32
Simultaneous.immobilization.and.formation.of.a.hydrogel.thin.ilm.on.gold.surfaces for.
cellular.micropatterning..(From.Y..Nakayama,.K..Nakamata,.Y..Hirano,.K..Goto,.and.T..Matsuda,.“Surface.
hydrogelation. of. thiolated. water-soluble. copolymers. on. gold,”.
Langmuir
. 14,. 3909-3915,. 1998..
Reprinted.with.permission.of.the.American.Chemical.Society..Figure.contributed.by.Takehisa.Matsuda.)
