Biomedical Engineering Reference
In-Depth Information
Shallow (2 µm) channels
filled by capillarity
Deep (10-100 µm) channels
continuosly perfused
Depletion of protein
Small patterned areas
~1 µm resolution
No depletion
Large areas
~10 µm resolution
100 μm
FIGURE 2.20
Capillary. versus. pressure-driven. low. in. microluidic. patterning. of. proteins.. (From.
Albert.Folch.and.Mehmet.Toner,.“Cellular.micropatterns.on.biocompatible.materials,”.
Biotechnol.
Prog.
.14,.388-392,.1998..Figure.contributed.by.the.author.)
(e) Example with one pair of antigen and antibody
(a) Immobilization
Channels
Antigen 1
Antigen-TRITC
Antigen 2
µFN
Substrate
Flow
(immobilization)
(b) Blocking
BSA
100 µm
(f )
Protein A dilutions
Reported
affinity
IgG
(c) Recognition
Chicken
Goat
Dog
Rabbit
Guinea pig
Human
None
Low
High
High
High
High
(d) Reading mosaic
Mouse (IgG
1
)
Low
Bovine
Low
50 µm
FIGURE 2.21
“Mosaic”.immunoassays.fabricated.by.microluidic.patterning..(From.A..Bernard,.B..
Michel,.and.E..Delamarche,.“Micromosaic.immunoassays,”.
Anal. Chem.
.73,.8,.2001..Reprinted.
with.permission.of.the.American.Chemical.Society..Figure.contributed.by.Emmanuel.Delamarche.)
