Summary of Methods Used in the Study of Pesticide Biotransformation and Disposition - Pesticide Biotransformation and Disposition - page 4

2

CHAPTER

Summary of Methods Used in the
Study of Pesticide Biotransformation
and Disposition

Chris Hofelt
North Carolina State University, Raleigh, NC, USA

Outline
Introduction

5

Analytical Methods for Pesticides and Pesticide Metabolites 6

Sampling 6

Blood 7

Urine and Bile 7

Tissue 8

Other Methods 8

Sample Extraction, Cleanup, and Enrichment 9

Thin-Layer Chromatography 10

Column Adsorption: Hydrophobic and Affinity 11

Size-Exclusion Chromatography 11

Enrichment 12

Analysis 12

Gas Chromatography 12

High-Performance Liquid Chromatography 13

Capillary Electrophoresis (CE) 13

Mass Spectroscopy 14

Uptake, Distribution, and Toxicokinetics 17

Cell Culture, Subcellular Fractions, and Recombinant Enzymes 18

Proteomics 20

Metabolomics 22

Summary 23

References 23

INTRODUCTION
Food security and public health contribute to the foundations of a prosperous civil
society. Pest management in general, and pesticides in particular, are of paramount
importance in contributing to this foundation. Rapidly emerging technologies have
contributed to the study of pesticide toxicology, allowing for the development of safer