Production of VNPs, VLPs, and Chimeras - Viral Nanoparticles: Tools for Materials Science and Biomedicine - page 53

Table 3.1 Summary of major expression systems for the production of VNPs and VLPs

Expression
system

Vector/inoculum Advantages Disadvantages Yields and

time frame

VNPs/VLPs

Plants (native
host)

Infected leaf material
Virus particles
DNA plasmid or

Intact particles
High yield
Ease of inoculation and purification
Inexpensive
Economic

Recombination events
can lead to deletions and
reversion to wild-type
progeny
Particles are infectious

1-2 g/1 kg
leaf material
2-3 weeks
1-2 months
to generate
mutants

BMV
CCMV
CPMV
HCSRV
RCNMV
TYMV
PVX
TMV

in

vitro transcripts

Prokaryotic
Escherichia
coli
Pseudomonas
fluorescens

Expression plasmid High yield

Commercial support (reagents and kits)
Inexpensive
Large-scale fermentation

Proteins frequently
insoluble
Coat proteins do not
always assemble into
particles
Expression of multiple
proteins challenging

1-2 g/1 l cell
culture
1-2 days

CCMV
TMV
MS2
Q

b
HK97
P22
T7

Eukaryotic
Yeast:
Saccharomyces
cerevisiae
Pichia pastoris

Expression plasmid Assembly of intact particles High yield

Ease of methods Commercial support
(reagents and kits) Inexpensive
Large-scale fermentation
Post-translational modifications

Expression of multiple
proteins is challenging
Cell lysis is difficult and
can lead to reduced yield

0.5 g/1 l cell
culture
1-2 days

BMV
CCMV
Q

b

Eukaryotic
Baculovirus

Recombinant
baculovirus

Assembly of intact VLPs
Commercial support (reagents and kits)
Multiple proteins can be expressed at the
same time
Moderate yields
Post-translational modifications

Expensive on a large scale 0.05 g/1 l

cell culture
Several days

FHV
CPV
CPMV

References are given throughout the text.