Playing “Nano -Lego ”: VNPs as Building Blocks for the Construction of Multi -Dimensional Arrays - Viral Nanoparticles: Tools for Materials Science and Biomedicine - page 173

Figure 7.3 (a) Structure of aniline. (b-d) Transmission electron microscopy images

of polyaniline/TMV composites synthesized after a 24-h reaction at (b) pH 6.5, (c, d)
pH 5.0; scale bar same for (b) and (d). Panels b-d were reproduced with permission
from Niu, Z., Bruckman, M. A., Li, S., Lee, L. A., Lee, B., Pingali, S. V., Thiyagarajan, P.,
and Wang, Q. (2007) Assembly of tobacco mosaic virus into fibrous and macroscopic
bundled arrays mediated by surface aniline polymerization, Langmuir , 23 (12),

6719-6724.

.. nanorings and Tripods

Complex 1D assemblies, such as nanorings and tripods have been created
using the M13 building block. 1D nanoring structures were constructed by
inserting two genetic modifications at each of the opposite ends of M13: a
streptavidin-binding peptide was expressed as pIII fusion and a hexa-His
tag was inserted in gene encoding pIX. The addition of a heterobifunctional
linker — a streptavidin-Ni-NTA complex — to the modified phages resulted
in reversible ring formation of the flexible filamentous particle (Fig. 7.4)
(Nam

et al ., 2004).