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Figure 5.7
Panel A: (a) Proposed mechanism of VLP assembly from coat proteins
(CP). First, electrostatic interaction leads to the formation of disordered protein-
gold nanoparticle complexes. The second step is a crystallization phase in which the
protein-protein interactions lead to the formation of a regular capsid. (b) Schematic
depiction of the encapsidated nanoparticle functionalized with carboxyl-terminated
TEG chains.
Panel B: Cryo-electron micrograph of a single VLP. The regular character
of the protein structure coating the 12 nm diameter gold nanoparticle (black disk)
is evident. The averages have been obtained by superposition of 10 individual
images, in each case. Panel C:
(a) Transmission electron micrograph of negatively
stained VLPs obtained from functionalized gold nanoparticles (black centers, 12 nm
diameter) and BMV capsid protein. (b) Comparison of encapsidation yields for citrate:
gold, PEG-gold, and native RNA. Averaged transmission electron micrograph of (c)
empty BMV capsid, (d) citrate-coated VLP, and (e) PEG-coated VLP. Reproduced with
permission from Chen, C., Daniel, M. C., Quinkert, Z. T., De, M., Stein, B., Bowman, V.
D., Chipman, P. R., Rotello, V. M., Kao, C. C., and Dragnea, B. (2006) Nanoparticle-
templated assembly of viral protein cages,
Nano Lett.
,
6
(4), 611-615.
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