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DNA-2) to a gold nanoparticle facilitated hybridization with RNA-1 resulting
in an OAS. The OAS-conjugated gold nanoparticles were mixed with RCNMV
coat protein monomers, and templated
self-assembly of RCNMV VLPs
around the gold core was facilitated (Fig. 5.5) (Loo
in vitro
., 2006).
This concept was expanded to achieve encapsulation of magnetic
nanoparticles and quantum dots (QDs) (Loo
et al
., 2006, 2007). Nanoparticles
with sizes of 5, 10, and 15 nm could be encapsulated. The size of the core
correlated with the VLP diameter (Fig. 5.6 and Table 5.1). Attempts to self-
assemble particles around 20 nm gold cores were not successful. The RCNMV
capsid has an exterior diameter of 36 nm, and the interior cavity has a
diameter of 17 nm (Sherman
et al
., 2006). Given the flexibility and dynamic
properties of viral coat proteins, it is interesting that they do not assemble
into larger structures when 20 nm-sized cores are provided.
et al
Figure 5.5
Assembly of RCNMV VLPs around a gold nanoparticles via OAS
templating. (a) Conjugation of nanoparticle with DNA-2; (b) addition of RNA-1
interacts with DNA-2 to form the functional OAS; (c) the artificial OAS templates
the assembly of coat protein; and (d) formation of virus-like particle with
nanoparticle encapsidated. Reproduced with permission from Loo, L., Guenther,
R. H., Lommel, S. A., and Franzen, S. (2007) Encapsidation of nanoparticles by red
clover necrotic mosaic virus,
J. Am. Chem. Soc.
,
129
(36), 11111-11117.
Figure 5.6.
Negatively stained transmission electron microscopy images of VLP
encapsidated (a) 4 nm CoFe
nano-
particles after purification. Reproduced with permission from Loo, L., Guenther,
R. H., Lommel, S. A., and Franzen, S. (2007) Encapsidation of nanoparticles by red
clover necrotic mosaic virus,
O
, (b) 10 nm CoFe
O
, and (f ) 15 nm CoFe
O
2
4
2
4
2
4
J. Am. Chem. Soc.
,
129
(36), 11111-11117.
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