The Art of Bioconjugation: Functionalization of VNPs - Viral Nanoparticles: Tools for Materials Science and Biomedicine - page 110

P22 Hexa-His tag (E;

GE)

Nanogold Non-covalent

Hexa-his-Ni-NTA
interaction

Proof-of-concept Kang et al .

(2008)

MS2 Tyr (I) Small

chemicals

Diazonium
coupling

Proof-of-concept Hooker et al .

(2004)

MS2 Cys (E; GE) Fluorescent

dye

Maleimide Proof-of-concept Peabody

(2003)

MS2 Lys (E) Fluorescent

dyes, stearic
acid

NHS ester,
pentafluophenyl
ester

Stabilization and
solubilization of
MS2 in organic
solvents

Johnson et

al . (2007)

MS2 Lys (E) Antibodies,

transferrin
(therapeutic
compounds
were
encapsulated)

NHS ester- and
maleimide-
reactive linkers

Targeted drug-
delivery

Brown et al .

(2002)

MS2 Lys (E) TAT (cell

penetrating
peptide),
(antisense
therapeutic
RNA was
encapsidated)

NHS ester- and
maleimide-
reactive linkers

Targeted RNA
delivery for gene
silencing

Wei et al .

(2009)

MS2 Tyr (I)

Lys (E)

Chelated
gadolinium
complexes

Isothiocyanate
or diazonium
coupling,
NHS-aldehyde
followed by oxime
condensation

Contrast agents
for MRI

Hooker et

al . (2007),

Datta et

al . (2008),

Anderson et

al . (2006)

MS2 Tyr (I) [ F]fluoro-

benzaldehyde
and fluorescent
dyes

18

Diazonium
coupling,
followed by oxime
condensation

Imaging
platforms
for positron
emission
tomography

Hooker et al .

(2008)

MS2 Tyr (I)

Lys (E)

Fluoresecent
dyes, biotin,
PEG-biotin,
PEG-dye
conjugates

Diazonium
NHS-aldehyde
followed by oxime
condensation

Development
of the MS2
platform for
potential
biomedical
applications

Kovacs et al .

(2007)

b

Q Lys (E) Fluorescent

dye,
bovine serum
albumin
(protein)

NHS-azide
followed by click
reaction

Optimized
click chemistry
protocol

Hong et al .

(2009)

Q b Azide (E; GE)

Alkyne (E; GE)

Fluorescent
dyes, chelated
gadolinium
complexes,
biotin

Click chemistry Imaging

modalities for
biomedical
applications

Strable et al .

(2008)