Biomedical Engineering Reference
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replica molding method, Li et al. manufactured grooves spaced from 1-2µm
apart with depths of 150-300nm on degradable PLLA [62]. PC-12 cells and chick
sympathetic neurons cultured onto these surfaces demonstrated neurite alignment
(up to 70%) as well as increased extension rates of 10-30%. Miller et al., used
laminin coated PLGA and PLDA with transferred microgrooves to demonstrate
95% alignment in DRG neurons [63]. Similarly, PC-12 cells grown on
PPFLMLLKGSTR (laminin-derived) functionalized PLGA microgrooves
showed neurite elongation parallel to the grooves. Neurites had a tendency to
reside within the channels and tighter pitched grooves improved angular
alignment [64].
Fig. 5. (A) Striped substrate configuration prior to coupling with p20 peptide. (B) Hippocampal
cells grew preferentially along the p20 coated regions. (C) Alternating stripes of laminin and
aggrecan microstamped onto a glass surface. (D) DRGs cultured onto laminin and aggrecan stripes
show preferred cell adhesion and orientation of laminin bands. (A), (B) Reprinted with permission
from [100]. (C),(D) Reprinted with permission from [101].
4.2.2.
Microchannel and microtubular architectures
Microtubular scaffolds for neural engineering have been an active research niche
because tubular arrays mimic the native endoneurial architecture in PNS. Ma and
Zhang first synthesized linear microchannels within PLLA and PLGA using a
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