Biomedical Engineering Reference
In-Depth Information
(C18/MPEG/API-g-PASPAM) and their hemolysis behavior were investigated to
evaluate their ensodomolytic ability. The structures of the two polymers are
shown in Figure 13 (a) and (b), respectively.
100
100
(a)
1mg/ml
2mg/ml
(b)
1mg/ml
2mg/ml
80
80
60
60
40
40
20
20
0
0
6.5
5.5
pH
7.4
pH
7.4
5.5
6.5
Fig. 14. Hemolysis by (a) MPEG/API-g-polyaspartamide and (b) C18/MPEG/API-g-
polyaspartamide as a function of pH after 1 hour incubation with 2w/v% red blood cell suspension.
Figure 14 (a) and (b) shows the results of hemolysis by (a) MPEG/API-g-
PASPAM and (b) C18/MPEG/API-g-PASPAM at various pHs. MPEG/API-g-
PASPAM showed no hemolysis activity with pH, but the agglutination of red
blood cells (RBCs) by polymers was observed and their agglutinate size
increased with decreasing pH from 7.4 to 5.5, as shown in Figure 15 (a) and (b).
Fig. 15. Images of RBC suspensions after incubation for 30 min in the presence of MPEG3-API97
at (a) pH 7.4 and (b) pH 5.5.
This agglutination phenomenon occurs due to the interaction between
negatively charged RBC membranes and positively charge polyaspartamides in
acidic environments and is related to the unique properties of the polymeric
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