Biomedical Engineering Reference
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coated with RGDSGGC. In addition, the authors showed enhanced affinity of
both chondrocytes and fibroblasts in a peptide-concentration dependent manner.
With increased RGDSGGC-chitosan concentration coated onto a microtiter plate,
chondrocyte and fibroblast cell adhesivity, as measured by optical density of the
crystal violet dye, was proportionally increased (Figure 7A, B), and fibroblast
proliferation also increased proportionally (Figure 7A, B).
3.2.2.
Case study: Peptide complexed hyaluronic acid
Another example of a peptide conjugated to a natural polymer material is the
RGD containing peptide sequence covalently linked to hyaluronic acid as
N
O
H
3
C
O
C
O
O
O
HO
O
O
O
O
OH
O
OH
OH
HO
O
HOH
2
C
O
O
OH
O
O
C
H
CO
2
-
Na
+
CH
3
n
n
(A)
(A)
IO
4
-
N
O
H
3
C
O
C
O
O
O
HO
O
O
O
O
OH
O
OH
OH
O
O
H
HOH
2
C
O
O
O
O
H
C
H
+
Na
-
O
2
C
O
CH
3
n
n
(B)
(B)
WH18
Peptide
NaCNBH
3
N
O
H
3
C
O
C
O
O
O
HO
O
O
O
O
OH
O
OH
OH
O
O
H
HOH
2
C
O
O
O
O
G
R
R
G
R
R
G
G
R
G
C
H
R
H
+
Na
-
O
2
C
CH
3
n
n
P
A
S
S
S
D
K
Fig. 8. Reaction of (A) diepoxide crosslinked hyaluronic acid with periodate to oxidize the
polyhydroxyls of the glucuronic acid to create reactive aldehydes and of (B) activated aldehydes of
the hyaluronic acid with the peptide sequence, followed by a reduction with sodium
cyanoborohydride to form a stable amine on the conjugation site [32].
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