Agriculture Reference
In-Depth Information
40
AFRICA
30
EUROPE
20
14
ASIA
10
6
4
13
3
1
2
5
7
12
AMERICA
8
9
11
10
0
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
Time (min)
Figure 3. Protein profiles obtained from one American (Harosoy), one Asian (Osage), one European
(Fred), and one African (Merit) soybean cultivars. Experimental conditions detailed in the text. Peaks
observed have been numbered from 1 to 14.
Results and Discussion
1. Separation of Soybean Proteins from Different Soybean Cultivars
A pool of 91 soybean cultivars with different origins have been analyzed with a reversed-
phase HPLC method already described [69]. As example, the chromatographic profiles of one
soybean cultivar from North America, one from Europe, one from Africa (Uganda), and one
from Asia are grouped in Figure 3. All soybean varieties showed 14 peaks consisting peak 13
of two unresolved peaks and being peaks 6 and 14 the majority ones in all cases. Despite
these similarities among the protein profiles observed for these soybean varieties, there were
some differences that could be useful for cultivar characterization. In addition to a different
peak size, some peaks were resolved in a different way such as peak 4 that was partially
resolved in two peaks in some cases. Table 1 shows the retention time and the average and
standard deviation of the area percentage of every peak in all American, European, Asian, and
the African cultivars studied. The only African cultivar employed (Merit original from
Uganda) presented peak area percentages very different to the observed for the other varieties,
especially for peaks 1, 2, 5, 10, 11, and 13. Peaks having the most differentiating capability
among American, Asian, and European soybeans were peaks 4, 10, and 13 while peaks
presenting the highest capability for the within group differentiation were peaks 1, 4, 7, and 9
(peaks presenting the highest RSD values).
In order to facilitate the interpretation of the data, multivariate classification methods
were applied to the area percentages obtained for every peak in each of the 91 soybean
cultivars by perfusion RP-HPLC.
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