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to the negative impact on the larvae weight, a similar toxicity was observed to Elite,
Conquista, Tucunaré and commercial soybeans when incorporated at a 16 % level, and
reductions as high as 88% could be observed (Figure 7A and Figure 8). Although some larvae
have survived in the seeds containing 16 % of the soybean seed coat flours, the number of
surviving larvae was drastically reduced for about 30 % (in artificial seeds containing
Conquista, Tucunaré and commercial soybean seed coat flours) or 10% in artificial seeds
containing seed coat flour from Elite cultivar (Figure 7B).
Figure 6. A - Oviposition and performance of Callosobruchus maculatus larvae on artificial seeds
covered with natural seed coats of cowpea (control seeds) and soybeans from commercial, Elite,
Conquista, Tucunaré, UFV Florestal, UFUS 2005 and UFUS 2005 cultivars. A' - Picure of artificial
seeds covered with natural seed coats. B - Mean mass of Callosobruchus maculatus larva which
survived on artificial seeds covered with natural seed coats. Experiments were done in triplicate and the
data shown are the average of these results. Bar = 1 cm.
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