Agriculture Reference
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digs the surface of the seed towards the cotyledons (Figure 4C); at the sixty day, we can
observe flour inside the egg giving a complete whitish aspect to the egg (Figure 4D), the larva
is partially inside of the cotyledon (Figure 4E), the seed coat is completely perforated (Figure
4F) as it is also perforated the cotyledon (Figure 4G).
Figure 4. Development of Callosobruchus maculatus larvae on natural Vigna unguiculata (cv. EPACE
10) seeds from the first day of oviposition to the sixth day of development. A - Egg at the first day; B -
Egg at the fifth day; C - Inferior surface of the egg (fifth day); D - Egg at the sixty day; E - Larva
penetrating the seed (sixty day); F - Hole across the seed coat (sixty day); G - Hole on the cotyledon
surface (sixty day). Bars= 0.5 mm. The bar inside panel A is equal for panels B-E.
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