Biology Reference
In-Depth Information
Fig. 2.
Schematic depiction of the ORF2 polyprotein of HAstV, and pro-
teolytic processing pathways proposed by different authors using different
serotypes (Geigenmüller
et al
., 2002).
8,22,24
Putative RNA binding domain
(RB) at the N-terminus of the polyprotein is indicated. Inverted arrowheads
denote putative cleavage sites dependent upon caspases, other unknown cel-
lular proteases (?), and trypsin (T). Underlined regions correspond to the
main viral epitopes identified by different authors,
24,37,38
being the epitopes
defined by the mAb 8E7 and 8G4 shared by all serotypes. The dotted line
indicates the two domains of the protein sequence (amino acids 1-415, well
conserved; amino acids 416-end, variable). White boxes represent interme-
diate products found within infected cells, while grey boxes represent the
final products present in infectious particles. See text for details. Amino acid
positions are numbered according to HAstV-1 Oxford reference strain
(accession no. L23513).
